Scarborough Athletic are out of the FA Trophy just days after exiting the FA Cup.
Fellow National North side Chorley edged through after a 24th minute winner.
Michael Coulson made his 250th appearance for the Seadogs in the loss.
Winger Harry Green suffered an ankle injury.
Boro boss Jono Greening told SAFC TV he felt aggrieved to suffer a 1-0 defeat:
The Seadogs return to league action on Tuesday night when King's Lynn visit the Flamingo Land Stadium in National North.
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